In Vitro Mammalian Cell Transformation Assays

MDS offers Transformation assays, which allows in vitro evaluation of carcinogenic potential using Syrian hamster embryo (SHE) cells or BALB/3T3 cells. The most frequently used endpoint for cell transformation is morphological transformation of mammalian cell fibroblasts in culture. Subsequent passage of these aberrant foci allows the expression of other characteristic phenotypes of malignant transformation, which include growth in soft agar and tumor formation in syngeneic hosts.

• Syrian Hamster Embryo (SHE) Transformation Assay
• BALB/3T3 Transformation Assay with and without S9 activation