Quantitative PCR Services: Host Cell DNA Contamination Assay
The determination of the amount of host cell DNA contamination in plasmid, viral, and protein preparations is a crucial piece of information for researchers and manufacturers alike. At MDS we offer two quantitative Real Time PCR assays based on host cell (E.Coli, or mammalian cell ) DNA.
E.Coli Host Cell DNA Contamination Assay
The E. coli host cell DNA contamination assay can be used to monitor host cell DNA removal during the manufacturing process and to assess host cell DNA contamination in the final product. In parallel with each assay, sample quality is assessed by an exogenous spike test to verify it is free of PCR inhibitors.
- Fully validated assay
- Rapid turnaround time of 1 to 2 weeks
- High throughput assay performed in 96 well format
- Highly sensitive - assay detects down to 1pg of E. coli DNA
- Wide quantitative range of typically up to 5 -7 log
- GLP testing available
- Mammalian Host Cell DNA Contamination Assay
- This assay is extremely flexible and can be used to detect contaminating host cell DNA from a wide range of cell lines derived from humans, mice, primates and hamsters. Sample quality is also determined using an exogenous spike test to verify sample is free of PCR inhibitors
- Detection of host cell DNA contamination in viral or protein preparations
- Assessment of host cell DNA damage using 3 different PCR primer sets
- Rapid turnaround time of 1 to 2 weeks
- High throughput assay performed in 96 well format
- High sensitivity - assay typically detects from 500fg of host cell DNA
- Wide quantitative range of typically up to 5-7 logs
- Qualified assay
- To place an order for Real-Q-Time PCR, please complete any/all of the pertinent submission forms under the forms tab above.