Cell Mediated Immunity
The stimulation of immune cells may act as a valuable marker of a therapeutic's efficacy and mechanism of action.
- Proliferation: mitogen stimulation assays for B and T cells (spleen)
- Epitope mapping
- Cytotoxicity
- Natural killer cell activity assay
- Receptor activation and cell signaling
- Apoptosis
- Cytokine production
- Opsonization/phagocytosis
- Immunostaining and flow-cytometric quantitation of spleen and lymph nodes for B cells and CD4+/CD8+ T cells
- Delayed-type hypersensitivity response to sheep or other species red blood cells
- Mixed leukocyte response against allogeneic leukocytes (MLR)
- Cytotoxic T lymphocyte (CTL) cytolysis against allogeneic tumor cells
- Kinetic evaluation of humoral response to T-dependent antigen (primary and secondary responses with sheep or chicken red blood cells)
- Kinetic evaluation of primary humoral response to a T-independent antigen (primary response with bacterial polysaccharides)